Baseline Correction for HPLC Chromatograms by Using Free Open-Source Software
نویسندگان
چکیده
Chromatograms with overlapping peaks and a baseline rise or upset constitute great challenge for analysts. Such case regarding the analysis of bupropion hydrochloride its 5 impurities in tablet formulation was used as model. A correction technique liquid chromatography coupled diode array detection is described by using Rstudio. The asymmetry least squares (ALS) algorithm implemented “baseline” package, parameters lambda p set to 4 0.05, respectively. Peak deconvolution subsequent integration area quantification were accomplished through Fytik software. Chromatographic data from validation procedure utilized demonstrate feasibility suggested method whether this affects outcome study. Finally, robustness study carried out order shed light on factors that have more significant influence correction, showing reliability random changes parameters.
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ژورنال
عنوان ژورنال: Analytica
سال: 2023
ISSN: ['2673-4532']
DOI: https://doi.org/10.3390/analytica4010005